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pc proteintech cat 16588 1 ap  (Proteintech)


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    Structured Review

    Proteintech pc proteintech cat 16588 1 ap
    Pc Proteintech Cat 16588 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pc/Pyruvate+Carboxylase+Antibody/pm41771860-580-59-60
    Average 94 stars, based on 54 article reviews
    pc proteintech cat 16588 1 ap - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Non-catalytic mechanisms of KMT5C regulating hepatic gluconeogenesis.
    Article Snippet: .. The following primary antibodies were used: anti-HSP90 (13171-AP), anti-PGC-1α (66369-1-Ig for western blot), anti-PC (16588-1-AP), antiFlag (66008-2-Ig), anti-α-Tubulin (66031-1-Ig), anti-Histone H4 (16047-1-AP), anti-Ubiquitin (10201-2-AP), from Proteintech; antiKMT5C (WH140428), anti-FOXO1 (A25985), anti-CREB1 (A11064), anti-PCK1 (A2036), anti-G6PC (A16234), anti-ACLY (A22273) from Abclonal; Anti-PGC-1α (NBP1-04676 for IP), anti-RNF34 (NBP2-56413) from Novus bio; anti-HA (sc-805X), anti-HNF-4α (sc-374229), antiCRTC2 (sc-271912) from Santa Cruz; anti-PPARα (ab314112) from abcam; anti-H4K20me3 (39672) from active motif. .. Secondary antibodies, anti-rabbit IgG (Vazyme, RA1008) and anti-mouse IgG (Vazyme, RA1009), were used for western blot following immunoprecipitation.

    Article Title: Compensatory activity of the PC-ME1 metabolic axis underlies differential sensitivity to mitochondrial complex I inhibition.
    Article Snippet: Cells were harvested in RIPA buffer (10mMTris-HCl pH8.0, 1mMEDTA, 1% Triton X-100, 0.1% SodiumDeoxycholate, 0.1% SDS, 140mMNaCl, 1X protease inhibitor cocktail, 1mM PMSF) and protein concentrations were quantified using the BCA assay (Pierce 23228). .. The following antibodies were used for western blot analysis: anti-ME1 (1/1000) (ab97445; Abcam); anti-G6PD (1/1000) (PA5-27359; Invitrogen); anti-PHGDH (1/ 1000) (14719-1-AP; Proteintech); anti-GFPT1 (1/1000) (14132-1-AP; Proteintech); anti-ACLY (1/1000) (15421-1-AP; Proteintech); anti MPC1 (1/ 1000) (14462; Proteintech); anti-PC (1/1000) (NBP-49536; Novus); antiIDH1 (1/1000) (#8137; Cell Signaling); anti-B III tubulin (1/1000) (ab78078; Abcam); anti-B actin (1/2000) (A5441; Sigma); anti-GFAP (1/ 1000) (ab7260; Abcam), anti-SMI-312 (1/500) (837904 BioLegend). ..

    Article Title: Non-catalytic mechanisms of KMT5C regulating hepatic gluconeogenesis
    Article Snippet: .. The following primary antibodies were used: anti-HSP90 (13171-AP), anti-PGC-1α (66369-1-Ig for western blot), anti-PC (16588-1-AP), anti-Flag (66008-2-Ig), anti-α-Tubulin (66031-1-Ig), anti-Histone H4 (16047-1-AP), anti-Ubiquitin (10201-2-AP), from Proteintech; anti-KMT5C (WH140428), anti-FOXO1 (A25985), anti-CREB1 (A11064), anti-PCK1 (A2036), anti-G6PC (A16234), anti-ACLY (A22273) from Abclonal; Anti-PGC-1α (NBP1-04676 for IP), anti-RNF34 (NBP2-56413) from Novus bio; anti-HA (sc-805X), anti-HNF-4α (sc-374229), anti-CRTC2 (sc-271912) from Santa Cruz; anti-PPARα (ab314112) from abcam; anti-H4K20me3 (39672) from active motif. .. Secondary antibodies, anti-rabbit IgG (Vazyme, RA1008) and anti-mouse IgG (Vazyme, RA1009), were used for western blot following immunoprecipitation.

    Article Title: Deletion of Pyruvate Carboxylase in Tubular Epithelial Cell Promotes Renal Fibrosis by Regulating SQOR/cGAS/STING‐Mediated Glycolysis
    Article Snippet: .. Primary antibodies for immunoblot analysis included rabbit anti‐PC (16588‐1‐AP, Proteintech, USA), rabbit anti‐Fibronectin (15613‐1‐AP, Proteintech, USA), rabbit anti‐COL1A1 (R10022, Zen‐bio, Chengdu, China), rabbit anti‐vimentin (10366‐1‐AP, Proteintech, USA), mouse anti‐α‐SMA (A5228, Sigma–Aldrich, USA), rabbit anti‐P‐SMAD3 (9520S, Cell Signaling Technology, USA), rabbit anti‐Snail (3879S, Cell Signaling Technology, USA), rabbit anti‐FIS1 (ab184554, Abcam, USA), rabbit anti‐SQOR (P02738, Hunan ProMab Biotechnologies, China), rabbit anti‐MNF2 (ab205236, Abcam, China), mouse anti‐Alpha Tubulin (66031‐1‐Ig, Proteintech, USA), and mouse anti‐ACTB/β‐actin (3700S, Cell Signaling Technology, China). .. The secondary antibodies used for immunoblot analysis were obtained from Jackson ImmunoResearch.

    Blocking Assay:

    Article Title: Glutathione peroxidase 4 (GPX4) and obesity interact to impact tumor progression and treatment response in triple negative breast cancer
    Article Snippet: Protein samples were resolved on 4-17.7% gradient polyacrylamide gels and transferred to nitrocellulose membranes (Bio-Rad). .. After blocking for 1 h in 5% non-fat milk, membranes were incubated overnight at 4 °C with anti-PC (1:1000, #16588-1-AP, Proteintech, Rosemont, IL), anti-β-actin, (1:5000, #sc-47778, Santa Cruz Biotechnology, Dallas, TX), anti-Nrf2 (1:500, #12721, Cell Signaling Technology, Danvers, MA), or anti-GPX4 (1:000, #144–01933, Raybiotech, Peachtree, GA) antibodies. .. Membranes were then incubated with secondary IRDye 680 RD goat anti-mouse (LI-COR #926-68070, 1:10,000) or IRDye 800CW goat anti-rabbit (LI-COR #926-32211, 1:10,000) antibody.

    Article Title: Glutathione peroxidase 4 (GPX4) and obesity interact to impact tumor progression and treatment response in triple negative breast cancer.
    Article Snippet: Protein samples were resolved on 4-17.7% gradient polyacrylamide gels and transferred to nitrocellulose membranes (Bio-Rad). .. After blocking for 1 h in 5% non-fat milk, membranes were incubated overnight at 4 °C with anti-PC (1:1000, #16588-1-AP, Proteintech, Rosemont, IL), anti-β-actin, (1:5000, #sc-47778, Santa Cruz Biotechnology, Dallas, TX), anti-Nrf2 (1:500, #12721, Cell Signaling Technology, Danvers, MA), or anti-GPX4 (1:000, #144–01933, Raybiotech, Peachtree, GA) antibodies. .. Membranes were then incubated with secondary IRDye 680 RD goat antimouse (LI-COR #926-68070, 1:10,000) or IRDye 800CW goat anti-rabbit (LI-COR #926-32211, 1:10,000) antibody.

    Incubation:

    Article Title: Glutathione peroxidase 4 (GPX4) and obesity interact to impact tumor progression and treatment response in triple negative breast cancer
    Article Snippet: Protein samples were resolved on 4-17.7% gradient polyacrylamide gels and transferred to nitrocellulose membranes (Bio-Rad). .. After blocking for 1 h in 5% non-fat milk, membranes were incubated overnight at 4 °C with anti-PC (1:1000, #16588-1-AP, Proteintech, Rosemont, IL), anti-β-actin, (1:5000, #sc-47778, Santa Cruz Biotechnology, Dallas, TX), anti-Nrf2 (1:500, #12721, Cell Signaling Technology, Danvers, MA), or anti-GPX4 (1:000, #144–01933, Raybiotech, Peachtree, GA) antibodies. .. Membranes were then incubated with secondary IRDye 680 RD goat anti-mouse (LI-COR #926-68070, 1:10,000) or IRDye 800CW goat anti-rabbit (LI-COR #926-32211, 1:10,000) antibody.

    Article Title: Glutathione peroxidase 4 (GPX4) and obesity interact to impact tumor progression and treatment response in triple negative breast cancer.
    Article Snippet: Protein samples were resolved on 4-17.7% gradient polyacrylamide gels and transferred to nitrocellulose membranes (Bio-Rad). .. After blocking for 1 h in 5% non-fat milk, membranes were incubated overnight at 4 °C with anti-PC (1:1000, #16588-1-AP, Proteintech, Rosemont, IL), anti-β-actin, (1:5000, #sc-47778, Santa Cruz Biotechnology, Dallas, TX), anti-Nrf2 (1:500, #12721, Cell Signaling Technology, Danvers, MA), or anti-GPX4 (1:000, #144–01933, Raybiotech, Peachtree, GA) antibodies. .. Membranes were then incubated with secondary IRDye 680 RD goat antimouse (LI-COR #926-68070, 1:10,000) or IRDye 800CW goat anti-rabbit (LI-COR #926-32211, 1:10,000) antibody.

    other:

    Article Title: The deubiquitinase OTUB1 inhibits gluconeogenesis by stabilizing YWHAB.
    Article Snippet: Hepatic gluconeogenesis plays a crucial role in maintaining glucose homeostasis and serves as a potential therapeutic target for type 2 diabetes, while its underlying mechanisms are not fully understood.. This study elucidates the role of the deubiquitinase OTU domain-containing ubiquitin aldehyde binding protein 1 (OTUB1) in gluconeogenesis.. We found that hepatic OTUB1 expression is reduced in both db/db mice and patients with type 2 diabetes.

    Immunohistochemistry:

    Article Title: Ganoderic acid T, a novel activator of pyruvate carboxylase, exhibits potent anti-liver cancer activity.
    Article Snippet: Background: Ganoderic acid T (GAT), a lanostane triterpenoid isolated from the methanol extract of G. lucidum mycelia, has demonstrated potent antitumor activity against various human cancer types.. However, the specific molecular targets of GAT in cancer cells remain largely unknown.. Therefore, this study systematically investigates these targets using the in vitro and in vivo hepatocellular carcinoma (HCC) model. Methods: The anti-tumor activities of GAT were validated in HCC cells, xenograft tumor models in nude mice, and patient-derived organoid models.

    Immunofluorescence:

    Article Title: Ganoderic acid T, a novel activator of pyruvate carboxylase, exhibits potent anti-liver cancer activity.
    Article Snippet: Background: Ganoderic acid T (GAT), a lanostane triterpenoid isolated from the methanol extract of G. lucidum mycelia, has demonstrated potent antitumor activity against various human cancer types.. However, the specific molecular targets of GAT in cancer cells remain largely unknown.. Therefore, this study systematically investigates these targets using the in vitro and in vivo hepatocellular carcinoma (HCC) model. Methods: The anti-tumor activities of GAT were validated in HCC cells, xenograft tumor models in nude mice, and patient-derived organoid models.

    Staining:

    Article Title: Ganoderic acid T, a novel activator of pyruvate carboxylase, exhibits potent anti-liver cancer activity.
    Article Snippet: Background: Ganoderic acid T (GAT), a lanostane triterpenoid isolated from the methanol extract of G. lucidum mycelia, has demonstrated potent antitumor activity against various human cancer types.. However, the specific molecular targets of GAT in cancer cells remain largely unknown.. Therefore, this study systematically investigates these targets using the in vitro and in vivo hepatocellular carcinoma (HCC) model. Methods: The anti-tumor activities of GAT were validated in HCC cells, xenograft tumor models in nude mice, and patient-derived organoid models.



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